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Table 2 Primers used for PCR detection of hemoparasites in wild Neotropical, Indo-Malayan and Australasian Psittaciformes

From: Can the intake of antiparasitic secondary metabolites explain the low prevalence of hemoparasites among wild Psittaciformes?

Gene

Primer name

Primer sequence 5’→3’

Size (bp)

Annealing

Parasite

Cytochrome b

Palu-Fq

CAAGGTAGCTCTAATCCTTTAGG

201

54 °C / 30 s

Haemoproteus; Plasmodium

Palu-R

DGGAACAATATGTARAGGAGT

Cytochrome b

L180

GAGAACTATGGAGTGGATGG

221

60 °C / 30 s

Leucocytozoon

Leunew1-R

CCCAGAAACTCATTTGWCC

18S rRNA

Try-F

GGAGAGGGAGCCTGAGAAATA

121

60 °C / 30 s

Trypanosoma

Try-R

ATGCACTAGGCACCGTCG

18S rRNA

NF110

GCTAATACATGCACCAAAGCTCC

119

60 °C / 30 s

microfilaria

NR228

CAAGACCATGCGATCAGC