Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole-susceptible and -resistant clones

Table 4 Phenotypic and genotypic characteristics of Fasciola hepatica clonal isolates

	No. of whole adult flukes recovered at PM	Mean weight of adult flukes ± SD (mg)	Median weight of adult flukes (mg)	Range of weights recorded for adult flukes (mg)	No. of adult flukes genotyped	No. of MLG observed
FhLivR1	70	108 ± 22	110	30–150	70	1^a
FhLivR1	165 (T)	83 ± 28	80	20–160	165	1^a
FhLivR2	31	86 ± 16.00	90	50–130	31	1^b
FhLivR2	48 (T)	98 ± 20	100	60–140	48	1^b
FhLivR3	35 (T)	102 ± 30	110	40–160	35	1
FhLivS1	15	177 ± 27	180	90–210	15	1
FhLivS1	0 (T)	–	–	–	–	–
FhLivS2	105^c	148 ± 25	150	80–230	110	1
FhLivS2	0 (T)	–	–	–	–	–
FhLivS3	112^d	129 ± 22	130	80–280	126	1
FhLivS3	0 (T)	–	–	–	–	–

^aSame genotype in both animals
^bSame genotype in both animals
^cPlus an additional 8 partial parasites
^dPlus an additional 15 partial parasites
Abbreviations: FhLiv clonal isolate of Fasciola, R resistant to triclabendazole, S susceptible to triclabendazole, (T) treated with 10mg/kg triclabendazole 10 days prior to PM, PM post-mortem, MLG multilocus genotype based on a panel of 15 microsatellite markers

ISSN: 1756-3305