Fig. 4

Heatmaps of expression of T. congolense genes encoding putative cell surface proteins. a GPI anchored proteins. Prediction of the putative GPI-anchored cell surface proteins was determined by FragAnchor [44], PredGPI [43] and BigPI [45]. b Transmembrane proteins. Trans-membrane helices was predicted using TMHMM [46]. The expression profiles consist of respective log₂ transformed RPKM clustered using Euclidean distance calculation and ward.D clustering methods. ¹Fam13/16 (VSGs), ²Fam50 (Brucei alanine-rich protein), ³Fam51 (Expression site-associated gene 4), ⁴Fam54 (Amino acid transporter), ⁵Fam56 (ABC transporter), ⁶Fam57 (Folate transporter, ESAG10), ⁷Fam60 (Membrane transporter protein), ⁸Fam61 (Nucleoside transporter), ⁹Fam67 (Cysteine peptidase), ¹⁰Fam12 (Procyclin-like), ¹¹Fam14 (Transferrin receptor-like, PAG-like)