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Fig. 5 | Parasites & Vectors

Fig. 5

From: Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans

Fig. 5

Expression analysis of selected CSP genes. a Expression levels of T. congolense gapdh from infected tsetse’s midgut, cardia and proboscis organs as well as bloodstream form parasites purified from infected mice blood. Abbreviations: BSF, bloodstream form; MG, tsetse midgut parasites; card, cardia parasites; PB, proboscis parasites. b Stage-regulated gene expression profiles for genes that putatively encode GPI-anchored and transmembrane proteins, normalized to gapdh levels as shown in A. * indicates transmembrane protein encoding genes. c Expression analysis of two GPI-anchored protein encoding genes via RT-qPCR. d Localization of T. congolense TcIL3000_0_02370 protein in parasites residing in the midgut, cardia and proboscis organs examined by immunofluorescent staining and confocal laser microscopy. Red indicates immunofluorescence staining of TcIL3000_0_02370, and blue indicates the DAPI staining of nucleus and kinetoplast

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