Antibody and cytokine response to Cystoisospora suis infections in immune-competent young pigs

Parasites & Vectors

Table 2 Targets and details of primers used for qPCR

Gene	Accession number	Primer sequence 5′-3′	Product size (bp)	Annealing temp. (°C)	Primer conc. (nM)	Primer efficiency (%)	Source
RPL4^a	XM_005659862.1	F: ATGCAAAGACAATGCGCCGA	127	60	400	101.7	This study
RPL4^a	XM_005659862.1	R: ACGGGCTTCTTGTCTGGAAC	127	60	400	101.7	This study
OAZ1^a	NM_001122994.1	F: CAATAGCTGCCTCTACATCGA	134	60	400	100.2	[27]
OAZ1^a	NM_001122994.1	R: GGTTCTTGTGGAAGCAAATGAAG	134	60	400	100.2	[27]
IFN-ɣ	NM_213948	F: GCTCTGGGAAACTGAATGAC	167	60	300	103.9	[75]
IFN-ɣ	NM_213948	R: TCTCTGGCCTTGGAACATAG	167	60	300	103.9	[75]
IL-12p35	NM_213993.1	F: CATGTGTCCGCTGCGCAACCT	98	69	250	99.5	[53]
IL-12p35	NM_213993.1	R: GCTGTGGTTGCAGGGAGGCTC	98	69	250	99.5	[53]
IL-2	NM_213861	F: GCCATTGCTGCTGGATTTAC	159	62	200	105.0	[75]
IL-2	NM_213861	R: CCCTCCAGAGCTTTGAGTTC	159	62	200	105.0	[75]
TNF-α	NM_214022	F: ACTGCACTTCGAGGTTATCGG	118	60	300	98.9	[76]
TNF-α	NM_214022	R: GGCGACGGGCTTATCTGA	118	60	300	98.9	[76]
IL-1β	NM_001005149	F: AGAAGAGCCCATCGTCCTTG	139	60	300	99.5	[75]
IL-1β	NM_001005149	R: GAGAGCCTTCAGCTCATGTG	139	60	300	99.5	[75]
IL-27	EST BP439244	F: GCCCGCCACTTTGCTGAATC	152	60	300	94.7	[75]
IL-27	EST BP439244	R: GGGCGAAGTGTCATGGAGAG	152	60	300	94.7	[75]
IL-6	NM_214399	F: ATCAGGAGACCTGCTTGATG	177	60	300	101.7	[75]
IL-6	NM_214399	R: TGGTGGCTTTGTCTGGATTC	177	60	300	101.7	[75]
IL-4	NM_214123.1	F: CAACCCTGGTCTGCTTACTG	173	60	300	91.9	[77]
IL-4	NM_214123.1	R: CTTCTCCGTCGTGTTCTCTG	173	60	300	91.9	[77]
IL-10	NM_214041	F: CCGAAGGCAGAGAGTGATGGG	111	60	300	103.9	[78]
IL-10	NM_214041	R: ACAGGGCAGAAATTGATGACAGC	111	60	300	103.9	[78]
TGF-β	NM_214015	F: GAAGCGCATCGAGGCCATTC	162	60	300	104.9	[75]
TGF-β	NM_214015	R: GGCTCCGGTTCGACACTTTC	162	60	300	104.9	[75]

^aReference genes for normalization
Abbreviations: F forward, R reverse, RPL4 ribosomal protein L4, OAZ ornithine decarboxylase antizyme, IFN interferon, IL interleukin, TGF transforming growth factor

ISSN: 1756-3305