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Table 1 Mosquito taxa collected in the Danube Delta Biosphere Reserve (Romania) in 2014 with information on the number of screened mosquito specimens, tested pools, Dirofilaria spp. screening results and estimated infection rates (EIR) per 100 mosquito specimens with corresponding 95% confidence intervals (95% CI)

From: Circulation of Dirofilaria immitis and Dirofilaria repens in the Danube Delta Biosphere Reserve, Romania

Mosquito species

No. of tested mosquito specimens

No. of tested mosquito pools

No. of mosquito pools positive for Dirofilaria spp. (% of tested pools)

No. of mosquito pools positive for D. immitis (% of tested pools)

EIR Dirofilaria immitis (95% CI)

No. of mosquito pools positive for D. immitis and D. repens (% of tested pools)

EIR D. repens (95% CI)

Coquillettidia richiardii

98,276

552

22 (3.99)

20 (3.62)

0.021 (0.013–0.032)

4 (0.72)

0.004 (0.001–0.010)

Anopheles hyrcanus

82,073

484

24 (4.96)

22 (4.55)

0.028 (0.018–0.041)

5 (1.03)

0.006 (0.002–0.014)

Culex pipiens (s.l.)/Cx. torrentium

18,421

217

9 (4.15)

9 (4.15)

0.051 (0.025–0.093)

0 (0.00)

0 (0.000–0.020)

Aedes caspius

13,729

197

9 (4.57)

7 (3.55)

0.053 (0.023–0.104)

3 (1.52)

0.022 (0.006–0.060)

Culex modestus

9534

166

0 (0.00)

0 (0.00)

0 (0.000–0.039)

0 (0.00)

0 (0.000–0.039)

Anopheles maculipennis (s.l.)

9380

207

20 (9.66)

17 (8.21)

0.206 (0.125–0.325)

6 (2.90)

0.066 (0.027–0.137)

Aedes vexans

7295

155

8 (5.16)

5 (3.23)

0.073 (0.027–0.164)

4 (2.58)

0.056 (0.018–0.134)

unidentified

1041

75

2 (2.67)

2 (2.67)

0.183 (0.036–0.576)

0 (0.00)

0 (0.000–0.315)

Anopheles algeriensis

697

34

1 (2.94)

1 (2.94)

0.126 (0.009–0.600)

0 (0.00)

0 (0.000–0.398)

Aedes spp.

71

12

1 (8.33)

0 (0.00)

0 (0.000–3.570)

1 (8.33)

1.263 (0.086–6.023)

Aedes detritus

31

5

0 (0.00)

0 (0.00)

0 (0.000–7.890)

0 (0.00)

0 (0.000–7.890)

Culex spp.

10

7

0 (0.00)

0 (0.00)

0 (0.000–25.441)

0 (0.00)

0 (0.000–25.441)

Aedes flavescens

5

2

0 (0.00)

0 (0.00)

0 (0.000–31.926)

0 (0.00)

0 (0.000–31.926)

Aedes cinereus

4

2

0 (0.00)

0 (0.00)

0 (0.000–39.179)

0 (0.00)

0 (0.000–39.179)

Aedes hungaricus

3

1

0 (0.00)

0 (0.00)

0 (0.000–40.888)

0 (0.00)

0 (0.000–40.888)

Culiseta annulata

1

1

0 (0.00)

0 (0.00)

0 (0.000–79.345)

0 (0.00)

0 (0.000–79.345)

Uranotaenia unguiculata

1

1

0 (0.00)

0 (0.00)

0 (0.000–79.345)

0 (0.00)

0 (0.000–79.345)

Sum resp. EIR (% of tested mosquito pools or 95% CI)

240,572

2118

96 (4.53)

83 (3.92)

0.036 (0.029–0.044)

23 (1.09)

0.010 (0.006–0.014)