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Fig. 2 | Parasites & Vectors

Fig. 2

From: Identification of Aedes aegypti cis-regulatory elements that promote gene expression in olfactory receptor neurons of distantly related dipteran insects

Fig. 2

CRE activity in the A. aegypti larval antenna. FAIRE DNA elements associated with the orco (a1), Or1 (b1, b2), fru (c1) and Or8 (d1, d2) genes promote GAL4 reporter expression in A. aegypti larval ORNs. The orco (a1) CRE promotes transgene expression in all larval ORNs, while the Or1 (b1, b2), fru (c1) and Or8 (d1, d2) CREs drive transgene expression in subsets of ORNs. These expression patterns are comparable to the patterns of native orco (a), Or1 (b), fru (c) and Or8 (d) transcripts in the larval antenna. CRE activity is comparable in two separate Or1-GAL4 lines (a in panel b1 and b in panel b2), as well as two separate Or8-GAL4 strains (a in panel d1 and b in panel d2). Mean gray value analyses for the cell marked by the yellow arrowheads in b1 versus b2 revealed no significant differences in transgene signal intensity levels (P > 0.05). Likewise, no differences in transgene signal intensity levels were detected for the cell marked by the blue arrowheads in d1 versus d2. Proximal is oriented upward in all panels

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Parasites & Vectors

ISSN: 1756-3305

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