Skip to main content
Fig. 3 | Parasites & Vectors

Fig. 3

From: Identification of Aedes aegypti cis-regulatory elements that promote gene expression in olfactory receptor neurons of distantly related dipteran insects

Fig. 3

CRE activity in A. aegypti adult antennal ORNs. Expression of GAL4 transcripts driven by FAIRE DNA elements adjacent to the fru (male in a1), orco (c1), Or1 (d1, d2) and Or8 (e1, e2) genes are comparable to expression of native fru (male in a), orco (c), Or1 (d) and Or8 (e) transcripts in the adult A. aegypti antenna. No fru transcript is detected in the A. aegypti female antenna (b), and GAL4 expression is not driven by the fru CRE in the female antenna (b1). CRE activity is comparable in two separate Or8-GAL4 lines (a in e1 and b in e2), as well as two separate Or1-GAL4 strains (a in panel d1 and b in panel d2). Mean gray value analyses for the cells marked by the yellow, blue, or purple arrowheads in d1 versus d2 revealed no significant differences in transgene signal intensity levels (P > 0.05). Likewise, no significant differences were detected in the transgene signal intensity levels of the cells marked by the magenta, cyan, or green arrowheads in e1 versus e2 (P > 0.05). The fru (male in a1), Or1 (d1, d2) and Or8 (e1 and e2) CREs are active in subsets of ORNs, while the orco (c1) CRE promotes gene expression in all ORNs. With the exception of a and a1, in which male antennae are shown, female antennae oriented proximal upward are displayed in all panels

Back to article page