Fig. 3From: PD-1 deficiency promotes TFH cells expansion in ITV-immunized mice by upregulating cytokines secretionPD-1 deficiency contributed to the expansion of TFR cells in both naïve and immunized mice. Splenocytes were isolated from immunized WT (n = 5) and PD-1-/- mice (n = 5) at the indicated times after the final immunization, and both the frequency and number of TFR cells were analyzed by FACS. a Representative FACS analysis of CD4+ICOS+CXCR5+Foxp3+CD19− cells. b, c Statistical analysis of the frequency and number of TFR cells from ITV-immunized WT and PD-1-/- mice on days 7, 14 and 21 after the final immunization. Three individual experiments were performed. The data are presented as the mean ± SD. Data were compared with the two-way ANOVA. Abbreviation: n, number of samplesBack to article page