Fig. 6

Frequency and number of GC B cells in the spleens of ITV-immunized WT and PD-1^-/- mice. Splenocytes were isolated from immunized WT (n = 5) and PD-1^-/- mice (n = 5) on days 7 and 14 after the final immunization and both the frequency and number of GC B cells were analyzed by FACS. a Representative FACS analysis of B220⁺GL-7⁺CD95⁺ GC B cells. b, c Statistical analysis of the frequency and number of GC B cells from ITV-immunized WT and PD-1^-/- mice. Three individual experiments were performed. The data are presented as means ± SD. Data were compared with two-way ANOVA. Abbreviation: n, number of samples