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Table 1 Viral sequences obtained by high throughput sequencing from sand flies pools captured in the rainy, intermediate and dry period at North Pantanal and Chapada dos Guimarães National Park, Mato Grosso, Brazil

From: Viola phlebovirus is a novel Phlebotomus fever serogroup member identified in Lutzomyia (Lutzomyia) longipalpis from Brazilian Pantanal

Place Period Pool Species (n) Reads Contigs Viral hits Genome Size (nt) Identity (%)a E value GenBank ID
North Pantanal Rainy 1 Evandromyia (Aldamyia) evandroi (5) 983,899,176 11,747
2 Lutzomyia sp. (25) 849,154.470 61,862
3 Ev. (Eva.) wilsoni (11) 819,202,516 43,993
4 Evandromyia (Aldamyia) carmelinoi (1) 1,179,702,220 91,115
5 Lu. (Tricholateralis) sherlocki (3) 449,698,864 28,893
6 Brumptomyia sp. (15) 785,799,190 73,656 Vesiculovirus ssRNA- 69 44.9 3.83e-12
Transitional 7 Lu. longipalpis (3) 645,865,104 13,763 Phlebovirus (Seg L) ssRNA- 6341 61 0 MF289183
Phlebovirus (Seg M) ssRNA- 4403 46 0 MF289182
Phlebovirus (Seg S) ssRNA- 1747 53 2e-79 MH119632
Dry 8 Lutzomyia sp. (5) 1,115,860,120 94,199 Vesiculovirus ssRNA- 72 40.3 4.10e-11
Flavivirus ssRNA+ 48 47.9 5.88e-10
CGNP Rainy 9 Lutzomyia sp. (22) 999,786,678 28,460
10 Nyssomyia whitmani (3) 787,599,818 37,990
11 Brumptomyia sp. (1)
Dry 12 Nyssomyia whitmani (12) 692,307,934 30,666
  1. aIdentity refers to the closest phlebovirus species Chagres virus indicated by BLASTx (GenBank)
  2. Abbreviations: CGNP Chapada dos Guimarães National Park, n number, nt nucleotides, Seg segment