Fig. 1

Melting temperatures and normalized melting profiles obtained with the HRM assays. a Melting temperatures (Tm) for all amplicons and standard species. For all amplicons 25 ng of genomic DNA was used as template. The plots show the mean, minimum and maximum of the Tm-values. Each species was tested in duplicate in three independent experiments. b Normalized HRM plots for all amplicons: (i), (ii) and (iii) represent the normalized melting profile for amplicon 1, amplicon 2 and amplicon 3, respectively. Each sample was tested in duplicate in three independent experiments. For (i), the bundles circled in the blue line, respectively from the left to the right, represent the profiles for L. (L.) tropica/L. (L.) major, L. (L.) donovani/L. (L.) infantum, L. (L.) amazonensis/L. (L.) mexicana, L. (V.) braziliensis, L. (V.) naiffi, L. (V.) guyanensis/L. (V.) shawi and L. (V.) lainsoni. For (ii), the bundles circled in the blue line, respectively from the left to the right, represent the profiles for L. (L.) amazonensis, L. (L.) mexicana, L. (L.) infantum, L. (L.) donovani, L. (L.) major and L. (L.) tropica. For (iii), bundles circled in the blue line, respectively from the left to the right, represent the profiles for L. (L.) infantum/L. (L.) major, L. (L.) tropica/L. (V.) lainsoni/L. (V.) naiffi, L. (L.) donovani/L. (V.) braziliensis/L. (V.) guyanensis/L. (V.) shawi, L. (L.) amazonensis, L. (L.) mexicana. Abbreviation: RFU: relative fluorescence units