Table 2 Primer sequences used in this study based on the amino acid permease 3 (aap3) coding sequence, for amplicon 1 (Amp1), amplicon 2 (Amp2) and amplicon 3 (Amp3)
Name | Sequence (5'-3') | Length (bp) | Orientation | Amplicon size (bp) |
|---|---|---|---|---|
AAP3-Amp1-F | ATCCGCTACGTCTCCGCCATCGG | 23 | Forward | 123 |
AAP3-Amp1-R | CGTGGTGAAGTACTTCATGTCGC | 23 | Reverse | |
AAP3-Amp2-F | GCCGTCGATAAACACCCGAGC | 21 | Forward | 131 |
AAP3-Amp2-R | AAGCGGAAGATGATGTTGCGCCC | 23 | Reverse | |
AAP3-Amp3-F | GGCGGTCGCCTACATCAGCG | 20 | Forward | 140 |
AAP3-Amp3-R | CGGGCACCATGAACACGAGCCATA | 24 | Reverse |