Table 4 Effect of initial amount of DNA on melting temperature. Mean and standard deviation (SD) for serial dilutions of DNA from standard strains. DNA concentration ranged from 25 ng to 100 fg. Human DNA concentration was kept constant at 25 ng/μl
Strain | Without human DNA | With human DNA |
|---|---|---|
Mean Tm ± SD (°C) | Mean Tm ± SD (°C) | |
Amplicon 1 | ||
 L. (L.) donovani | 82.52 ± 0.10 | 82.64 ± 0.18 |
 L. (L.) mexicana | 83.16 ± 0.10 | 83.23 ± 0.13 |
 L. (V.) braziliensis | 83.31 ± 0.09 | 83.43 ± 0.11 |
 L. (V.) guyanensis | 83.83 ± 0.08 | 83.96 ± 0.34 |
Amplicon 2 | ||
 L. (L.) donovani | 85.44 ± 0.14 | 85.44 ± 0.14 |
 L. (L.) mexicana | 84.40 ± 0.08 | 84.56 ± 0.07 |
 L. (L.) infantum | 84.92 ± 0.11 | 85.17 ± 0.14 |
 L. (L.) tropica | 86.33 ± 0.13 | 86.73 ± 0.18 |
Amplicon 3 | ||
 L. (L.) donovani | 86.17 ± 0.12 | 86.15 ± 0.12 |
 L. (L.) mexicana | 86.77 ± 0.16 | 86.91 ± 0.16 |
 L. (V.) braziliensis | 86.00 ± 0.14 | 86.17 ± 0.15 |
 L. (V.) guyanensis | 85.97 ± 0.13 | 86.36 ± 0.13 |