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Table 4 Effect of initial amount of DNA on melting temperature. Mean and standard deviation (SD) for serial dilutions of DNA from standard strains. DNA concentration ranged from 25 ng to 100 fg. Human DNA concentration was kept constant at 25 ng/μl

From: Amino acid permease 3 (aap3) coding sequence as a target for Leishmania identification and diagnosis of leishmaniases using high resolution melting analysis

Strain Without human DNA With human DNA
Mean Tm ± SD (°C) Mean Tm ± SD (°C)
Amplicon 1
L. (L.) donovani 82.52 ± 0.10 82.64 ± 0.18
L. (L.) mexicana 83.16 ± 0.10 83.23 ± 0.13
L. (V.) braziliensis 83.31 ± 0.09 83.43 ± 0.11
L. (V.) guyanensis 83.83 ± 0.08 83.96 ± 0.34
Amplicon 2
L. (L.) donovani 85.44 ± 0.14 85.44 ± 0.14
L. (L.) mexicana 84.40 ± 0.08 84.56 ± 0.07
L. (L.) infantum 84.92 ± 0.11 85.17 ± 0.14
L. (L.) tropica 86.33 ± 0.13 86.73 ± 0.18
Amplicon 3
L. (L.) donovani 86.17 ± 0.12 86.15 ± 0.12
L. (L.) mexicana 86.77 ± 0.16 86.91 ± 0.16
L. (V.) braziliensis 86.00 ± 0.14 86.17 ± 0.15
L. (V.) guyanensis 85.97 ± 0.13 86.36 ± 0.13