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Table 5 Identification of Leishmania in naturally and experimentally infected samples by HRM analysis targeting the aap3 gene

From: Amino acid permease 3 (aap3) coding sequence as a target for Leishmania identification and diagnosis of leishmaniases using high resolution melting analysis

Sample source HRM identification Previous diagnosis
Amplicon 1 Amplicon 2 Amplicon 3 Diagnostic method Species identification
Humana L. (L.) amazonensis negative negative SSU rDNA sequencing L. (L.) amazonensis
Humanb L. (L.) infantum L. (L.) infantum L. (L.) infantum SSU rDNA sequencing L. (L.) infantum
Catc L. (L.) infantum L. (L.) infantum L. (L.) infantum hsp70 HRM L. (L.) infantum
Moused L. (L.) amazonensis L. (L.) amazonensis L. (L.) amazonensis SSU rDNA sequencing L. (L) amazonensis
Moused L. (V.) braziliensis negative L. (V.) braziliensis g6pd PCR L. (V.) braziliensis
Sand fliese L. (L.) infantum L. (L.) infantum L. (L.) infantum SSU rDNA sequencing L. (L.) infantum
Sand fliesf Subgenus Viannia negative Subgenus Viannia g6pd PCR L. (V.) braziliensis
  1. Note: The aap3 amplicons 1, 2 and 3 of DNA from each sample was submitted to HRM analysis. The result was compared with previous identification performed by SSU rDNA-sequencing [26], hsp70 HRM [14] or g6pd PCR [31]
  2. aHuman paraffin-embedded tissue from Hospital das Clínicas de São Paulo
  3. bHuman paraffin-embedded tissue from Irmandade da Santa Casa de Misericórdia de São Paulo
  4. cIsolated parasites from cat
  5. dExperimentally infected BALB/c mice
  6. eNaturally infected Lutzomyia (Lutzomyia) longipalpis
  7. fNaturally infected Lu. (Nyssomyia) whitmani