Parasites & Vectors

Table 5 Identification of Leishmania in naturally and experimentally infected samples by HRM analysis targeting the aap3 gene

From: Amino acid permease 3 (aap3) coding sequence as a target for Leishmania identification and diagnosis of leishmaniases using high resolution melting analysis

Sample source	HRM identification			Previous diagnosis
Sample source	Amplicon 1	Amplicon 2	Amplicon 3	Diagnostic method	Species identification
Human^a	L. (L.) amazonensis	negative	negative	SSU rDNA sequencing	L. (L.) amazonensis
Human^b	L. (L.) infantum	L. (L.) infantum	L. (L.) infantum	SSU rDNA sequencing	L. (L.) infantum
Cat^c	L. (L.) infantum	L. (L.) infantum	L. (L.) infantum	hsp70 HRM	L. (L.) infantum
Mouse^d	L. (L.) amazonensis	L. (L.) amazonensis	L. (L.) amazonensis	SSU rDNA sequencing	L. (L) amazonensis
Mouse^d	L. (V.) braziliensis	negative	L. (V.) braziliensis	g6pd PCR	L. (V.) braziliensis
Sand flies^e	L. (L.) infantum	L. (L.) infantum	L. (L.) infantum	SSU rDNA sequencing	L. (L.) infantum
Sand flies^f	Subgenus Viannia	negative	Subgenus Viannia	g6pd PCR	L. (V.) braziliensis

Note: The aap3 amplicons 1, 2 and 3 of DNA from each sample was submitted to HRM analysis. The result was compared with previous identification performed by SSU rDNA-sequencing [26], hsp70 HRM [14] or g6pd PCR [31]
^aHuman paraffin-embedded tissue from Hospital das Clínicas de São Paulo
^bHuman paraffin-embedded tissue from Irmandade da Santa Casa de Misericórdia de São Paulo
^cIsolated parasites from cat
^dExperimentally infected BALB/c mice
^eNaturally infected Lutzomyia (Lutzomyia) longipalpis
^fNaturally infected Lu. (Nyssomyia) whitmani

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ISSN: 1756-3305

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