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Table 1 Primer and probe sequences for the 10 kinds of pathogen

From: Establishment and optimization of a liquid bead array for the simultaneous detection of ten insect-borne pathogens

Pathogen Primer or probe sequence Amplified fragment length (bp) GenBank ID
ASFV Forward primer: 5'-AGTTATGGGAAACCCGACCC-3' 254 FN557520
Reverse primer: Biotin-5'-CCCTGAATCGGAGCATCCT-3'
Capture probe: NH2-C12-5'-AGCCTTATGTTCCAGTAGGGTTTG-3'
RC-probe: Biotin-5'-CAAACCCTACTGGAACATAAGGCT-3'
BTV Forward primer: 5'-GCAGCATTTTGAGAGAGCGA-3' 101 KP 822059.1
Reverse primer: Biotin-5'-CCCGATCATACATTGCTTCCT-3'
Capture probe: NH2-C12-5'-GGAGGAGCCGGCGAAAGCATA-3'
RC-probe: Biotin-5'-TATGCTTTCGCCGGCTCCTCC-3'
BB Forward primer: 5'-AAGAGCAGACGGAACCAGAC-3' 345 AY502600
Reverse primer: Biotin-5'-GTGCCATTTGAGTCGTATTG-3'
Capture probe: NH2-C12-5'-AATTCAGGCACTTCAACTTTAACAA-3'
RC-probe: Biotin-5'-TTGTTAAAGTTGAAGTGCCTGAATT-3'
EHDV Forward primer: 5'-TGATTATGATGTTCATGGCGAA-3' 189 KU140939.1
Reverse primer: Biotin-5'-ACCTTGGAGCTTCACTCTATCT-3'
Capture probe: NH2-C12-5'-TAGATGGATTTGACATACCGCCGGA-3'
RC-probe: Biotin-5'-TCCGGCGGTATGTCAAATCCATCTA-3'
VSV Forward primer: 5'-TGGACGGGCTTGAAAATCAGTGCAAA-3' 199 KP 202364.1
Reverse primer: Biotin-5'-TTGAATCTGGAAACAATAGTTCCGTATCT-3'
Capture probe: NH2-C12-5'-ATGTTCTTCCACATGTTCAAAAA-3'
RC-probe: Biotin-5'-TTTTTGAACATGTGGAAGAACAT-3'
WNV Forward primer: 5'-GAGCCACTCAGGCAGGGAGATTCAG-3' 496 KY229074.1
Reverse primer: Biotin-5'-AACACCACAGTGCCGTGACCT-3'
Capture probe: NH2-C12-5'-TGAAGGGAACAACCTATGGCGTCTG-3'
RC-probe: Biotin-5'-CAGACGCCATAGGTTGTTCCCTTCA-3'
CB Forward primer: 5'-TGAGATTCGGGGGTTGTTGC-3' 376 M80806
Reverse primer: Biotin-5'-ACACCTCCTTATTCCCACTCG-3'
Capture probe: NH2-C12-5'-TTTAACGGCGCTCTCGGTTTATGCG-3'
RC-probe: Biotin-5'-CGCATAAACCGAGAGCGCCGTTAAA-3'
RVFV Forward primer: 5'-ATGATGACATTAGAAGGGA-3' 298 DQ380149
Reverse primer: Biotin-5'-ATGCTGGGAAGTGATGAG-3'
Capture probe: NH2-C12-5'-ATGCTGTAGTTCCAAACTCAGCCCT-3'
RC-probe: Biotin-5'-AGGGCTGAGTTTGGAACTACAGCAT-3'
EBV Forward primer: 5'-CGCTGGCTGGTGTTAATGTAGGG-3' 249 KC545392.1
Reverse primer: Biotin-5'-ATGCAGTCGTGATGGCTTCGG-3'
Capture probe: NH2-C12-5'-CAGCAAACTAACGCAATGGTAACCT-3'
RC-probe: Biotin-5'-AGGTTACCATTGCGTTAGTTTGCTG-3'
SBV Forward primer: 5'-GAAGCTAGTGCTCAGATTGTCATGC-3' 130 KC545392.1
Reverse primer: Biotin-5'-GTGGATAGAAGTCAAAAGCATCAAGG-3'
Capture probe: NH2-C12-5'-AAGGGATGCACCTGGGCCGATGGTTA-3'
RC-probe: Biotin-5'-TAACCATCGGCCCAGGTGCATCCCTT-3'
  1. Abbreviations: ASFV African swine fever virus, BTV bluetongue virus, BB Borrelia burgdorferi, EHDV epizootic hemorrhagic disease virus, VSV vesicular stomatitis virus, WNV West Nile virus, CB Coxiella burnetii, RVFV Rift Valley fever virus, EBV Ebola virus, SBV Schmallenberg virus