Table 1 Primer and probe sequences for the 10 kinds of pathogen

ASFV

Forward primer: 5'-AGTTATGGGAAACCCGACCC-3'

254

FN557520

Reverse primer: Biotin-5'-CCCTGAATCGGAGCATCCT-3'

Capture probe: NH₂-C₁₂-5'-AGCCTTATGTTCCAGTAGGGTTTG-3'

RC-probe: Biotin-5'-CAAACCCTACTGGAACATAAGGCT-3'

BTV

Forward primer: 5'-GCAGCATTTTGAGAGAGCGA-3'

101

KP 822059.1

Reverse primer: Biotin-5'-CCCGATCATACATTGCTTCCT-3'

Capture probe: NH₂-C₁₂-5'-GGAGGAGCCGGCGAAAGCATA-3'

RC-probe: Biotin-5'-TATGCTTTCGCCGGCTCCTCC-3'

BB

Forward primer: 5'-AAGAGCAGACGGAACCAGAC-3'

345

AY502600

Reverse primer: Biotin-5'-GTGCCATTTGAGTCGTATTG-3'

Capture probe: NH₂-C₁₂-5'-AATTCAGGCACTTCAACTTTAACAA-3'

RC-probe: Biotin-5'-TTGTTAAAGTTGAAGTGCCTGAATT-3'

EHDV

Forward primer: 5'-TGATTATGATGTTCATGGCGAA-3'

189

KU140939.1

Reverse primer: Biotin-5'-ACCTTGGAGCTTCACTCTATCT-3'

Capture probe: NH₂-C₁₂-5'-TAGATGGATTTGACATACCGCCGGA-3'

RC-probe: Biotin-5'-TCCGGCGGTATGTCAAATCCATCTA-3'

VSV

Forward primer: 5'-TGGACGGGCTTGAAAATCAGTGCAAA-3'

199

KP 202364.1

Reverse primer: Biotin-5'-TTGAATCTGGAAACAATAGTTCCGTATCT-3'

Capture probe: NH₂-C₁₂-5'-ATGTTCTTCCACATGTTCAAAAA-3'

RC-probe: Biotin-5'-TTTTTGAACATGTGGAAGAACAT-3'

WNV

Forward primer: 5'-GAGCCACTCAGGCAGGGAGATTCAG-3'

496

KY229074.1

Reverse primer: Biotin-5'-AACACCACAGTGCCGTGACCT-3'

Capture probe: NH₂-C₁₂-5'-TGAAGGGAACAACCTATGGCGTCTG-3'

RC-probe: Biotin-5'-CAGACGCCATAGGTTGTTCCCTTCA-3'

CB

Forward primer: 5'-TGAGATTCGGGGGTTGTTGC-3'

376

M80806

Reverse primer: Biotin-5'-ACACCTCCTTATTCCCACTCG-3'

Capture probe: NH₂-C₁₂-5'-TTTAACGGCGCTCTCGGTTTATGCG-3'

RC-probe: Biotin-5'-CGCATAAACCGAGAGCGCCGTTAAA-3'

RVFV

Forward primer: 5'-ATGATGACATTAGAAGGGA-3'

298

DQ380149

Reverse primer: Biotin-5'-ATGCTGGGAAGTGATGAG-3'

Capture probe: NH₂-C₁₂-5'-ATGCTGTAGTTCCAAACTCAGCCCT-3'

RC-probe: Biotin-5'-AGGGCTGAGTTTGGAACTACAGCAT-3'

EBV

Forward primer: 5'-CGCTGGCTGGTGTTAATGTAGGG-3'

249

KC545392.1

Reverse primer: Biotin-5'-ATGCAGTCGTGATGGCTTCGG-3'

Capture probe: NH₂-C₁₂-5'-CAGCAAACTAACGCAATGGTAACCT-3'

RC-probe: Biotin-5'-AGGTTACCATTGCGTTAGTTTGCTG-3'

SBV

Forward primer: 5'-GAAGCTAGTGCTCAGATTGTCATGC-3'

130

KC545392.1

Reverse primer: Biotin-5'-GTGGATAGAAGTCAAAAGCATCAAGG-3'

Capture probe: NH₂-C₁₂-5'-AAGGGATGCACCTGGGCCGATGGTTA-3'

RC-probe: Biotin-5'-TAACCATCGGCCCAGGTGCATCCCTT-3'