Establishment and optimization of a liquid bead array for the simultaneous detection of ten insect-borne pathogens

Table 3 Analysis of positive samples using the optimized liquid array detection system and corresponding reference methods or nucleic acid detection kits

Pathogens	Results of the two methods		Description of reference method or nucleic acid detection kit
Pathogens	Optimized liquid array detection system	Reference methods or nucleic acid detection kit
ASFV	0	0	PCR and agarose gel electrophoresis (primers recommended by OIE)
BTV	53	53	BTV nucleic acid detection kit^a
BB	16	16	Borrelia burgdorferi nucleic acid detection kit^b
EHDV	0	0	Virus isolation
VSV	0	0	VSV RT-PCR detection kit^c
WNV	0	0	WNFV real-time PCR detection kit^d
CB	17	17	Isolation of pathogen
RVFV	0	0	RVFV nucleic acid detection kit^e
EBV	0	0	EBV nucleic acid detection kit^f
SBV	0	0	Virotype SBV real-time RT-PCR kit(FLI-B585)^g

^aBTV nucleic acid detection kits and the real-time fluorescence quantitative PCR method were purchased from Beijing Senkang Biotechnology Development Co., Ltd., Beijing, China
^bBorrelia burgdorferi nucleic acid detection kits and the PCR-fluorescent probe method were purchased from Guangzhou Vipotion Biotechnology Co. Ltd., Guangzhou, China
^cVSV RT-PCR detection kits were purchased from YUABIO Biotechnology Co., Beijing, China
^dWNFV real-time PCR detection kits were purchased from Beijing Biolab Technology Co. Ltd.
^eRVFV nucleic acid detection kits and the real-time fluorescence quantitative PCR method were purchased from Beijing Senkang Biotechnology Development Co., Ltd., Beijing, China
^fEBV nucleic acid detection kits and the PCR-fluorescent probe method were purchased from DA AN GENE Co. Ltd., Guangzhou, China
^gVirotype SBV real-time RT-PCR kit (FLI-B585) was purchased from Qiagen, Valencia, CA, USA

ISSN: 1756-3305