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Table 3 Analysis of positive samples using the optimized liquid array detection system and corresponding reference methods or nucleic acid detection kits

From: Establishment and optimization of a liquid bead array for the simultaneous detection of ten insect-borne pathogens

Pathogens Results of the two methods Description of reference method or nucleic acid detection kit
Optimized liquid array detection system Reference methods or nucleic acid detection kit
ASFV 0 0 PCR and agarose gel electrophoresis (primers recommended by OIE)
BTV 53 53 BTV nucleic acid detection kita
BB 16 16 Borrelia burgdorferi nucleic acid detection kitb
EHDV 0 0 Virus isolation
VSV 0 0 VSV RT-PCR detection kitc
WNV 0 0 WNFV real-time PCR detection kitd
CB 17 17 Isolation of pathogen
RVFV 0 0 RVFV nucleic acid detection kite
EBV 0 0 EBV nucleic acid detection kitf
SBV 0 0 Virotype SBV real-time RT-PCR kit(FLI-B585)g
  1. aBTV nucleic acid detection kits and the real-time fluorescence quantitative PCR method were purchased from Beijing Senkang Biotechnology Development Co., Ltd., Beijing, China
  2. bBorrelia burgdorferi nucleic acid detection kits and the PCR-fluorescent probe method were purchased from Guangzhou Vipotion Biotechnology Co. Ltd., Guangzhou, China
  3. cVSV RT-PCR detection kits were purchased from YUABIO Biotechnology Co., Beijing, China
  4. dWNFV real-time PCR detection kits were purchased from Beijing Biolab Technology Co. Ltd.
  5. eRVFV nucleic acid detection kits and the real-time fluorescence quantitative PCR method were purchased from Beijing Senkang Biotechnology Development Co., Ltd., Beijing, China
  6. fEBV nucleic acid detection kits and the PCR-fluorescent probe method were purchased from DA AN GENE Co. Ltd., Guangzhou, China
  7. gVirotype SBV real-time RT-PCR kit (FLI-B585) was purchased from Qiagen, Valencia, CA, USA