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Fig. 3 | Parasites & Vectors

Fig. 3

From: Pike intestinal reaction to Acanthocephalus lucii (Acanthocephala): immunohistochemical and ultrastructural surveys

Fig. 3

Immunohistochemistry of mast cells (MCs) of E. lucius parasitized intestine. a Immunoreactivity to anti-FCεRIγ is localized to plasmalemma or regions of peripheral cytoplasm of MCs (arrows). b Micrograph showing numerous mast cells in lamina propria-submucosa; some MCs positive for piscidin 3 are evident (arrows). c Most MCs are immunoreactive to the anti-histamine antibody (arrows). d Anti-lysozyme antibody is detected in MCs of the pike intestine. A rodlet cell (arrowhead) can be seen in the epithelium. e MCs positive to the anti-serotonin antibody in the epithelium (thin arrows) and in the lamina propria-submucosa (thick arrows). In the epithelium, the anti-serotonin antibody detects an endocrine cell (arrowhead). f MCs immunopositive to the anti-interleukin-6 antibody (arrows) scattered among collagen fibres of the lamina propria-submucosa. g Several MCs immunopositive to the anti-TNF-α antibody (thick arrows). Some of them are in the epithelium (thin arrow). h Anti-met-enkephalin antibody detects MCs (arrows) among the collagen fibres of the stratum compactum. i MCs immunoreactive to the anti-substance P antibody in the lamina propria-submucosa (thick arrows) and in the epithelium (thin arrows) of the pike intestine. j Negative control section showing no immunoreactivity in the lamina propria-submucosa at point of parasite (P) attachment to the pike intestine Abbreviation: e, epithelium. Scale-bars: a, c-f, 20 μm; b, 10 μm; g-j, 50 μm

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