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Table 1 Antibodies used in the present study, their species specificity, source and dilution. The slides were incubated for 24 h at room temperature for each antibody. Slides treated with anti-TNF-α antibody were previously heated by 2 × 5 min microwave cycles at 500 W in 0.01 M citrate buffer pH 6.0 for unmasking antigene, as indicated in the data sheet of this antibody

From: Pike intestinal reaction to Acanthocephalus lucii (Acanthocephala): immunohistochemical and ultrastructural surveys

Antibody anti- Specificity Source Code Dilution
Immunoglobulin E (IgE)-like receptor FcεRIγ Mouse, monoclonal Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA sc-390222 1:50
Histamine Rabbit, polyclonal Sigma-Aldrich, Saint Louis, MO, USA H7403 1:50
Interleukin-1beta Mouse, monoclonal Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA sc-32294 1:50
Interleukin-6 Mouse, monoclonal Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA sc-28343 1:50
Lysozyme Rabbit, polyclonal Dako Glostrup, Denmark (now part of Agilent, Santa Clara, CA, USA) A0099 1:100
Met-Enkephalin Rabbit, polyclonal Millipore, Burlington, MA, USA AB1975 1:100
NOS 2 (i-NOS) Rabbit, polyclonal Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA sc-651 1:10
Piscidin-3 (HAGR) Rabbit, monoclonal Bethyl Laboratories, Montgomery, TX, USA 1:400
Serotonin Rabbit, polyclonal Chemicon (now part of Merck Millipore, Darmstadt, Germany) AB938 1:100
Substance P Rabbit, polyclonal Peninsula Labs., Belmont, CA, USA T4170 1:200
Tumor necrosis factor (TNF-α) Rabbit, polyclonal Abcam, Cambridge, UK ab6671 1:100