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Table 1 Antibodies used in the present study, their species specificity, source and dilution. The slides were incubated for 24 h at room temperature for each antibody. Slides treated with anti-TNF-α antibody were previously heated by 2 × 5 min microwave cycles at 500 W in 0.01 M citrate buffer pH 6.0 for unmasking antigene, as indicated in the data sheet of this antibody

From: Pike intestinal reaction to Acanthocephalus lucii (Acanthocephala): immunohistochemical and ultrastructural surveys

Antibody anti-

Specificity

Source

Code

Dilution

Immunoglobulin E (IgE)-like receptor FcεRIγ

Mouse, monoclonal

Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA

sc-390222

1:50

Histamine

Rabbit, polyclonal

Sigma-Aldrich, Saint Louis, MO, USA

H7403

1:50

Interleukin-1beta

Mouse, monoclonal

Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA

sc-32294

1:50

Interleukin-6

Mouse, monoclonal

Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA

sc-28343

1:50

Lysozyme

Rabbit, polyclonal

Dako Glostrup, Denmark (now part of Agilent, Santa Clara, CA, USA)

A0099

1:100

Met-Enkephalin

Rabbit, polyclonal

Millipore, Burlington, MA, USA

AB1975

1:100

NOS 2 (i-NOS)

Rabbit, polyclonal

Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA

sc-651

1:10

Piscidin-3 (HAGR)

Rabbit, monoclonal

Bethyl Laboratories, Montgomery, TX, USA

–

1:400

Serotonin

Rabbit, polyclonal

Chemicon (now part of Merck Millipore, Darmstadt, Germany)

AB938

1:100

Substance P

Rabbit, polyclonal

Peninsula Labs., Belmont, CA, USA

T4170

1:200

Tumor necrosis factor (TNF-α)

Rabbit, polyclonal

Abcam, Cambridge, UK

ab6671

1:100