Table 1 Primers used in the study. The restriction sites are underlined
Primer ID | Primer sequence 5'-3' |
|---|---|
Hc-clec-160 1F | ATGCAACCCCTCTGTACCTT |
Hc-clec-160 1R | TTAGATGACAGGACAGTAGTGGC |
Hc-clec-160 2F | GGATCCATGCAACCCCTCTGTACCTT |
Hc-clec-160 2R | AAGCTTTTAGATGACAGGACAGTAGTGGC |
Hc-clec-160 3F | TCTAGAATGCAACCCCTCTGTACCTT |
Hc-clec-160 3R | GGTACCATGACAGGACAGTAGTGGC |
Hc-clec-160 4F | TCTAGAATGCAACCCCTCTGTACCTT |
Hc-clec-160 4R | AAGCTTTTAGATGACAGGACAGTAGTGGC |
Ce-clec-160 pF | CCGGACCATCAATATGGTATTGAGTAT |
Ce-clec-160 pR | CTGAATAATTCTTGGGTATTAAAAAAAAGA |
Ce-clec-160 pF1 | GTCGACCCGGACCATCAATATGGTATTGAGTAT |
Ce-clec-160 pR1 | TCTAGACTGAATAATTCTTGGGTATTAAAAAAAAGA |
Hc-clec-160 QF | AGTAGCGGCTTTGATTGTGC |
Hc-clec-160 QR | TGACTGCTGACCCATTGCTT |
Ce-clec-160 F | CCCGGGATGGATTTAAAAAGTTGGATTCTA |
Ce-clec-160 R | AAGCTTCTAATTGCTTGAAGGTGCACAGTA |
Ce-clec-160 QF | AGTTCCACCTACACATCCG |
Ce-clec-160 QR | AAGTCCACCTTGTCCCATT |