Skip to main content
Fig. 4 | Parasites & Vectors

Fig. 4

From: Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages

Fig. 4

HTB partially affects PGE2 production and COX-2 expression by activated macrophages. Macrophages were pre-incubated overnight with HTB (1000 nM) and stimulated with LPS plus IFN-γ (10 ng/ml of each). The PGE2 production was measured after 24 h in cell-free supernantants by ELISA (a). The expression of COX-2 was evaluated after 6 h in cell lysates by Western blot (b). The expression of COX-2 and β-actin was determined by densitometry analysis of the bands. The results are presented as a percentage of the control, which corresponds to unstimulated cells (c). The data represent the mean ± SEM. *P < 0.05 versus control

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us