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Fig. 6 | Parasites & Vectors

Fig. 6

From: Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages

Fig. 6

HTB does not affect lymphocyte proliferation or cytokine production. Lymphocytes were pre-incubated overnight with HTB (125 to 1000 nM) and stimulated with Con A (0.5 μg/ml, final concentration). After 72 h, the proliferation was measured by a colorimetric assay (a) and the levels of IFN-γ (b), IL-4 (c), IL-5 (d) and IL-10 (e) were measured in cell-free supernatants by ELISA. The data represent the mean ± SEM. *P < 0.05 versus control and #P < 0.05 versus LPS/IFN-γ-activated cells

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