Fig. 6From: Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophagesHTB does not affect lymphocyte proliferation or cytokine production. Lymphocytes were pre-incubated overnight with HTB (125 to 1000 nM) and stimulated with Con A (0.5 μg/ml, final concentration). After 72 h, the proliferation was measured by a colorimetric assay (a) and the levels of IFN-γ (b), IL-4 (c), IL-5 (d) and IL-10 (e) were measured in cell-free supernatants by ELISA. The data represent the mean ± SEM. *P < 0.05 versus control and #P < 0.05 versus LPS/IFN-γ-activated cellsBack to article page