Fig. 1From: Characterization of a novel secretory spherical body protein in Babesia orientalis and Babesia orientalis-infected erythrocytesPCR amplification of the BoSBP4 gene from B. orientalis gDNA and linear pGEX-6P-1 fragment from pGEX-6P-1 plasmid. Lane M: marker; Lane 1: amplificon from gDNA; Lane 2: negative control; Lane 3: amplificon from gDNA for recombinant plasmid construction; Lane 4: amplificon from plasmid of pGEX-6P-1 for recombinant plasmid construction. The corresponding bands are indicated by arrowsBack to article page