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Fig. 5 | Parasites & Vectors

Fig. 5

From: Characterization of a novel secretory spherical body protein in Babesia orientalis and Babesia orientalis-infected erythrocytes

Fig. 5

Western blot identification of immunoreactivity and native form of BoSBP4. a Lane M: molecular weight marker; Lane 1: rBoSBP4 probed with the serum of B. orientalis-infected buffalo; Lane 2: rBoSBP4 probed with the serum of uninfected buffalo. b Identification of the native form of BoSBP3 in B. orientalis merozoite lysate. Lane M: molecular weight marker; Lane 1: reaction of lysate of B. orientalis-infected erythrocytes with the serum against rBoSBP4; Lane 2: reaction of lysate of uninfected buffalo erythrocytes with the serum against rBoSBP4; Lane 3: lysate of B. orientalis-infected buffalo erythrocytes probed with anti-GST tag mouse monoclonal antibody; Lane 4: lysate of B. orientalis-infected buffalo erythrocytes probed with the serum of naïve mouse; Lane 5: lysate of uninfected buffalo erythrocytes probed with the serum of naïve mouse. The corresponding bands are indicated by arrows

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