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Fig. 2 | Parasites & Vectors

Fig. 2

From: Exposure to amitraz, fipronil and permethrin affects cell viability and ABC transporter gene expression in an Ixodes ricinus cell line

Fig. 2

IRE/CTVM19 cells after treatment with 0.5% DMSO (a), 150 μM amitraz (b), 150 μM fipronil (c) and 150 μM permethrin (d), stained with modified May Grünwald-Giemsa (Diff-Quik) stain. Drug-treated cultures showed increased cellular debris, less well-defined cell borders and, in the case of fipronil, vacuolated nuclei (white arrows). Cells in mitosis are indicated by black arrows. Pictures were captured with brightfield illumination and a 25× objective using an Axio Imager M2 microscope with Axiocam MRc camera and Zen software (Zeiss, Milan, Italy). Scale-bars: 50 μm

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