Uranotaenia unguiculata Edwards, 1913 are attracted to sound, feed on amphibians, and are infected with multiple viruses

Table 4 Summary of infection and potential transmission of West Nile virus lineage 4c and Alphamesonivirus 1 by Uranotaenia unguiculata mosquitoes

Virus	Sample^a	Total	Pools^b	Positive pools^c
West Nile virus	Body	362	44	9
	Legs and wings	225	39	6
	Honey card	-	9	1
Alphamesonivirus 1	Body	362	38	3
	Legs and wings	11	11	2
	Honey card	-	9	3

^aMosquitoes were provided a 25% honey solution on Whatman® FTA® cards, then dissected, removing legs and wings from the body
^bLegs and wings were stored separately from body, and samples were pooled into tubes of 2–50 (bodies) or 1–10 (legs and wings). Each honey card sampled between 20-50 mosquitoes
^cPools were tested for the presence of virus by RT-PCR

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