Table 1 Primers used to amplify the seven MEP pathway enzyme genes of Babesia orientalis
Primer | Sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|
DXS-F | ATGGAGTTGTGTTGTAATC | 2094 |
DXS-R | TTAGGATGCAAGGAATTGG | Â |
DXR-F | ATGAATGCAGCAGTGAGTTTTTATG | gDNA 1554 |
DXR-R | TTAGTATGTGAAGCATTAATATATGTGATAG | cDNA 1371 |
IspD-F | ATGTCTGTCTGGTTGGTGCAATG | 1344 |
IspD-R | TCACGGGAAGTATACCTCTTTTAG | Â |
IspE-F | ATGAGGCATCTCCATGTGTG | 1521 |
IspE-R | TCAAGAGATTAGGTCTGGAAGAAG | Â |
IspF-F | ATGTTACTACGTTATCTTTCTATAACAG | 654 |
IspF-F | TTATAAAGGATATTACTTGAGCTTC | Â |
IspG-F | ATGACATCGTCAGACACCTTTG | 1932 |
IspG-R | TCACATGCTTCCACATCTGG | Â |
IspH-F | ATGGAGGAAAGACCGTTTACC | 1056 |
IspH-R | TTATGTAGTTTTCCAGATTCCTATAATCG | Â |