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Table 2 Light microscopy findings by infection group and time point compared to uninfected NC group

From: Mutual interactions of the apicomplexan parasites Toxoplasma gondii and Eimeria tenella with cultured poultry macrophages

  2 hpi 24 hpi 48 hpi 72 hpi
  EH EL CI EH EL CI EH EL CI EH EL CI
Attached macrophages +++ +++ +++ +++ +++ ++ +++ +++ ++ ++ ++ +
Macrophage vacuolization - - + ++ + ++ + + +++ ++ ++ +++
Free Eta sporozoites + + + - - - - - - - - -
Intracellular Et a sporozoites + + + + + + - - - - - -
Intracellular meronts - - - + + ++ ++ ++ ++ ++ ++ ++
Et a merozoites - - - - - - - - - + + -c
  TH TL CI TH TL CI TH TL CI TH TL CI
Attached macrophages +++ +++ +++ ++ +++ ++ + ++ ++ - + +
Macrophage vacuolization ++ + + ++ + ++ +++ ++ +++ - +++ +++
Free Tgb tachyzoites +++ ++ ++ + - - +++ + + + +++ +++
Intracellular Tgb tachyzoites + + + ++ + + + ++ ++ + ++ +++
  1. Findings were scored semiquantitatively (-, not observed; +, low amounts; ++, moderate amounts; +++, high amounts). Infection groups: TH, high-dose infection with T. gondii; TL, low-dose infection with T. gondii; EH, high-dose infection with E. tenella; EL, low-dose infection with E. tenella, CI co-infected group.
  2. aEt, E. tenella
  3. bTg, T. gondii
  4. cNot clearly observable due to the numerous replication of T. gondii tachyzoites presented in cell culture by light microscopy