Skip to main content

Advertisement

Table 1 Gene-specific primers used in this study. Italics denotes RNA polymerase promoter sequences

From: Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis

Primer Sequence [5'→3']
HlGST real-time forward CTTCTTGGATCTTGGCGGGT
HlGST real-time reverse CGATGTCCCAGTAGCCGAG
HlGST RT forward ACGTGAAGCTCACCCAGAGCAT
HlGST RT reverse AAGCTAGCCATGTCGCCGTTGA
HlGST RNAi forward GCCTGGCTCAAGGAGAAACACA
HlGST RNAi reverse ACAAAGGCCTTCAGGTTGGGGA
HlGST T7 forward TAATACGACTCACTATAGGGCCTGGCTCAAGGAGAAACACA
HlGST T7 reverse TAATACGACTCACTATAGGACAAAGGCCTTCAGGTTGGGGA
HlGST2 real-time forward CCCTTCCGGGAATGAAGGAG
HlGST2 real-time reverse GATCGCTCAGCAGTCGTCAG
HlGST2 RT forward ACGTCAAGCTGACGCAGAGCAT
HlGST2 RT reverse ATGGGCCAAGCCTTGAAGCGAT
HlGST2 RNAi forward AGGATAAAAGGTACGGCTTCGGCA
HlGST2 RNAi reverse TTTCACGATCTGGAGAGCCTCGTA
HlGST2 T7 forward TAATACGACTCACTATAGGAGGATAAAAGGTACGGCTTCGGCA
HlGST2 T7 reverse TAATACGACTCACTATAGGTTTCACGATCTGGAGAGCCTCGTA
P0 real-time forward CTCCATTGTCAACGGTCTCA
P0 real-time reverse TCAGCCTCCTTGAAGGTGAT
L23 real-time forward CACACTCGTGTTCATCGTCC
L23 real-time reverse ATGAGTGTGTTCACGTTGGC
Actin real-time forward ATCCTGCGTCTCGACTTGG
Actin real-time reverse GCCGTGGTGGTGAAAGAGTAG
Actin RT forward CCAACAGGGAGAAGATGACG
Actin RT reverse ACAGGTCCTTACGGATGTCC
Tubulin real-time forward TTCAGGGGCCGTATGAGTAT
Tubulin real-time reverse TGTTGCAGACATCTTGAGGC
EGFP T7 forward TAATACGACTCACTATAGGGACGTAAACGGCCACAAGTT
EGFP T7 reverse TAATACGACTCACTATAGGTGCTCAGGTAGTGGTTGTCG