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Table 2 Enzyme kinetic constants of recombinant GSTs in the presence of different acaricides

From: Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis

Acaricide Class Recombinant HlGST Recombinant HlGST2
V max K m Inhibition V max K m Inhibition
None   11.70 ± 1.92 0.82 ± 0.14   14.72 ± 0.56 0.61 ± 0.20  
Flumethrin Synthetic pyrethroids 4.26 ± 0.30* 0.48 ± 0.07* UC 5.85 ± 0.73 1.70 ± 0.92 None
Cypermethrin Synthetic pyrethroids 3.61 ± 1.65* 0.38 ± 0.04* UC 2.20 ± 0.64* 0.36 ± 0.12 NC
Chlorpyrifos Organophosphates 9.52 ± 4.21 0.55 ± 0.24 None 8.00 ± 1.08* 0.35 ± 0.25 NC
Ethion Organophosphates 8.36 ± 0.87 0.58 ± 0.15 None 15.55 ± 1.02 0.37 ± 0.07 None
Coumaphos Organophosphates 6.35 ± 2.40 0.48 ± 0.10* None 9.68 ± 3.38 1.17 ± 0.31 None
Diazinon Organophosphates 18.38 ± 5.43 1.51 ± 0.78 None 26.13 ± 5.33 1.59 ± 0.58 None
Amitraz Formamidine 15.04 ± 1.66 0.87 ± 0.25 None 10.10 ± 2.55 0.51 ± 0.09 None
Ivermectin Avermectin 10.94 ± 5.10 0.74 ± 0.36 None 14.64 ± 3.47 0.74 ± 0.31 None
  1. Abbreviations: UC uncompetitive inhibition, NC noncompetitive inhibition
  2. *P < 0.05 vs no acaricide