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Table 3 Diversity of tick-borne microorganisms in Ixodes ricinus and Haemaphysalis concinna ticks infesting free-living ungulates. Values represent numbers of positive/examined tick samples and prevalence (in %)

From: Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia

Tick/Host

Capreolus capreolus

Cervus elaphus

Dama dama

Ovis musimon

Sus scrofa

(i) Ixodes ricinus

 Larvae

Anaplasma phagocytophilum

25/31 (27.7)a; 4b

5/6 (23.0)a; 3b

24/32 (22.0)a; 11b

5/5 (57.1)a; 4b

 

Rickettsia sp.

14/31 (11.6)a

 

1/32 (0.6)a

  

Rickettsia helvetica

1/31 (0.7)a

1/6 (3.1)a

3/32 (1.7)a

  

Rickettsia monacensis

  

2/3 (21.2)a

  

Coxiella burnetii

  

1/32 (0.6)a

  

Babesia venatorum

3/31 (2.1)a

    

Theileria sp.

19/31 (17.7)a; 7b

1/6 (3.1)a; 1b

11/32 (8.3)a; 8b

  

Borrelia valaisiana

1/31 (0.75)a

    

No. of animals infested with larvae

7

3

13

4

0

 Nymphs

Anaplasma phagocytophilum

30/59 (50.9); 5b

12/15 (80.0); 4b

62/101 (61.4); 9b

1/1 (100); 1b

 

Rickettsia sp.

2/59 (3.4)

1/15 (6.7)

   

Rickettsia helvetica

2/59 (3.4)

 

8/101 (7.9)

  

Coxiella burnetii

4/59 (6.8)

    

CNM

1/59 (1.7)

    

Theileria sp.

17/59 (28.8); 7b

9/15 (60.0); 1b

22/101 (21.8); 10b

  

Borrelia valaisiana

  

1/101 (1.0)

  

Borrelia afzelii

1/59 (1.7)

    

Borrelia garinii

1/59 (1.7)

 

1/101 (1.0)

  

No. of animals infested with nymphs

9

4

12

1

1

 Females

Anaplasma phagocytophilum

2/3 (66.7); 1b

7/7 (100); 4b

54/58 (93.1); 14b

4/4 (100); 3b

 

Rickettsia helvetica

  

8/58 (13.8)

1/4 (25.0)

 

Coxiella burnetii

  

1/58 (1.7)

1/4 (25.0)

 

CNM

2/3 (66.7)

 

1/58 (1.7)

  

Babesia venatorum

  

1/58 (1.7)

  

Theileria sp.

2/3 (66.7); 2b

3/7 (42.9); 3b

39/58 (67.2); 11b

  

No. of animals infested with females

2

4

15

3

0

 Males

Anaplasma phagocytophilum

1/1 (100); 1b

2/3 (66.7); 1b

13/16 (81.2); 7b

1/1 (100); 1b

 

Rickettsia sp.

1/1 (100)

    

Rickettsia helvetica

  

1/16 (6.2)

  

Theileria sp.

0/1 (0); 0

1/3 (33.3); 1b

8/16 (50.0); 4b

  

Borrelia valaisiana

  

1/16 (6.2)

  

No. of animals infested with males

1

1

8

1

0

(ii) Haemaphysalis concinna

 Larvae

Anaplasma phagocytophilum

2/12 (16.7); 2b

 

2/6 (33.3); 1b

1/1 (100); 1b

 

Babesia sp.

2/12 (16.7)

  

1/1 (100)

 

No. of animals infested with larvae

5

0

2

1

0

 Nymphs

     

Theileria sp.

5/14 (35.7); 2b

    

No. of animals infested with nymphs

2

0

2

0

0

  1. Abbreviation: CNM, “Candidatus Neoehrlichia mikurensis”
  2. aMLE, maximum likelihood estimation of infection prevalence
  3. bNumbers of A. phagocytophilum- or Theileria sp.-positive ungulates from which infected ticks were collected