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Fig. 5 | Parasites & Vectors

Fig. 5

From: Characterization of a serine protease inhibitor from Trichinella spiralis and its participation in larval invasion of host’s intestinal epithelial cells

Fig. 5

IIF analysis of binding between rTsSPI and IECs (× 200). rTsSPI, IL1 ES antigens or PBS were used for pre-incubating with IEC for 2 h at 37 °C. rTsSPI was also pre-incubated with C2C12 for 2 h at 37 °C. After washes, the pre-incubated IEC and C2C12 was probed by anti-rTsSPI serum, infection serum or pre-immune serum, subsequently colored with goat anti-mouse IgG-FITC conjugate. Propidium iodide (PI) dyed cell nuclei in red

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