Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia

Table 1 List of primers used in PCR assays

Organism	Target gene	Primer	Oligonucleotide sequence (5′-3′)	Amplicon size (bp)	Annealing T (°C)	Reference
B. bigemina	hyp	BiIA	CATCTAATTTCTCTCCATACCCCTCC	278	63	[26]
		BiIB	CCTCGGCTTCAACTCTGATGCCAAAG	278	63
		BiIAN	CGCAAGCCCAGCACGCCCCGGTGC	170	70
		BiIBN	CCGACCTGGATAGGCTGTGTGATG	170	70
B. bovis	RAP-1	BoF	CACGAGGAAGGAACTACCGATGTTGA	350	61	[26]
		BoR	CCAAGGAGCTTCAACGTACGAGGTCA	350	61
		BoFN	TCAACAAGGTACTCTATATGGCTACC	290	67
		BoRN	CTACCGAGCAGAACCTTCTTCACCAT	290	67
Babesia spp.	18S rRNA	A	ACCTGGTTGATCCTGCCAG	~1700	60	[30]
Babesia spp.	18S rRNA	B	GATCCTTCTGCAGGTTCACCTAC	~1700	60	[30]
B. bigemina	18S rRNA	Bbig200F	GCGTTTATTAGTTCGTTAACC	~1200	56	[32]
B. bigemina	18S rRNA	Bbig1400R	ACAGGACAAACTCGATGGATGC	~1200	56	[32]
B. bovis	18S rRNA	AN	GCTTGTCTTAAAGATTAAGCCATGC	~1550	60	[31]
B. bovis	18S rRNA	BN	CGACTTCTCCTTCCTTTAAGTGATAAG	~1550	60	[31]
Mammalian	cyt b	Cyt bF	CCCCTCAGAATGATATTTGTCCTCA	~383	60	[28]
Mammalian	cyt b	Cyt bR	CCATCCAACATCTCAGCATGATGAAA	~383	60	[28]
Insects	cox1	LCOI490	GGTCAACAAATCATAAAGATATTGG	~710	45	[29]
Insects	cox1	HCO2198	TAAACTTCAGGGTGACCAAAAAATCA	~710	45	[29]

ISSN: 1756-3305