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Fig. 2 | Parasites & Vectors

Fig. 2

From: The marsupial trypanosome Trypanosoma copemani is not an obligate intracellular parasite, although it adversely affects cell health

Fig. 2

Microscopy of Trypanosoma cruzi infecting potoroo kidney epithelial (PtK2) cells. a Light micrograph of Diff-Quik stained cells and parasites after 96 h incubation showing a single cell with amastigotes inside, as recognisable by their round nucleus and disc shaped kinetoplast (arrowhead). b Heavily infected area of PtK2 cells (arrowhead) following incubation with T. cruzi for 120 h and stained with Diff-Quik. c Scanning electron micrograph of T. cruzi infecting PtK2 cells. The dying cell membranes were broken fortuitously exposing developing T. cruzi amastigotes (arrowhead) inside. d Transmission electron micrograph of T. cruzi amastigotes developing inside PtK2 cells. T. cruzi is recognisable by the dense elongate kinetoplasts (arrowheads). Scale-bars: a, 10 μm; b, 20 μm; c, 2 μm; d, 1 μm

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