Primer | Sequence (5'-3') | Position | Product size (bp) | Annealing T (°C) |
---|---|---|---|---|
SDNP amplification | ||||
SDNP-RTPCR-FP | AAGACTTGCAACTCCATCTCGAT | 219 | 170 | 60 |
SDNP-RTPCR-RP | ATTTTCTCGCTGACCACCACAACT | 366 | ||
S. digitata actin amplification (reference gene) | ||||
SDACT-RTPCR-FP | CGCTCGAGAAATCTTACGAATTGC | 707 | 147 | 55 |
SDACT-RTPCR-RP | TCCTTTCTGATATCGATATCACATT | 854 |