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Table 2 Primers used for qPCR. The primer sequences of the target (SDNP) and internal control (Actin) are given

From: Functional analysis of a novel parasitic nematode-specific protein of Setaria digitata larvae in Culex quinquefasciatus by siRNA mediated RNA interference

Primer

Sequence (5'-3')

Position

Product size (bp)

Annealing T (°C)

SDNP amplification

SDNP-RTPCR-FP

AAGACTTGCAACTCCATCTCGAT

219

170

60

SDNP-RTPCR-RP

ATTTTCTCGCTGACCACCACAACT

366

S. digitata actin amplification (reference gene)

SDACT-RTPCR-FP

CGCTCGAGAAATCTTACGAATTGC

707

147

55

SDACT-RTPCR-RP

TCCTTTCTGATATCGATATCACATT

854

  1. Abbreviation: T temperature