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Table 3 Cytokine profile after incubation of lymphocytes from the spleen with different doses of concanavalin A, ConA (0 μg/ml; 5 μg/ml)

From: Changes in the immune system in experimental acanthamoebiasis in immunocompetent and immunosuppressed hosts

Parameter/group   Group A (n = 10) Group C (n = 6) Group AS (n = 10) Group CS (n = 6) Kruskal-Wallis test
IL-4
 8 dpi ConA0 0 0 0 0 P = 1.0
ConA5 0.52 ± 4.33 0.26 ± 0.59 0 0 H = 2.16, df = 3, P = 0.54
 16 dpi ConA0 0 0 0 1.94 ± 4.33 H = 3.18, df = 3, P = 0.39
ConA5 50.35 ± 51.75a 0 0a 0 H = 11.22, df = 3, P = 0.002
 24 dpi ConA0 0 0 1.28 ± 2.86 0 H = 3.00, df = 3, P = 0.31
ConA5 1.19 ± 2.67 0.66 ± 1.47 0 0 H = 21.00, df = 3, P = 0.54
IL-10
 8 dpi ConA0 84.63 ± 46.72 149.98 ± 128.88 83.06 ± 37.32 58.90 ± 26.14 H = 3.94, df = 3, P = 0.27
ConA5 168.43 ± 180.88 212.92 ± 180.20 139.47 ± 125.42 86.16 ± 12.56 H = 4.07, df = 3, P = 0.25
 16 dpi ConA0 128.27 ± 65.83 78.98 ± 58.82 78.60 ± 46.47 69.07 ± 15.19 H = 4.62, df = 3, P = 0.34
ConA5 511.09 ± 40.41b, c 67.42 ± 16.98b 276.18 ± 224.72c,d 81.01 ± 19.34d H = 10.30, df = 3, P = 0.01
 24 dpi ConA0 14.94 ± 33.40 19.99 ± 99.96 0 0 H = 2.16, df = 3, P = 0.55
ConA5 82.13 ± 82.73 36.54 ± 74.12 0 230.67 ± 515.76 H = 3.73, df = 3, P = 0.29
IL-17 A
 8 dpi ConA0 0 0 0 0 P = 1.0
ConA5 13.86 ± 30.98 0 0 0 H = 3.00, df = 3, P = 0.39
 16 dpi ConA0 0 0 0 0.75 ± 1.68 H = 4.00, df = 3, P = 0.56
ConA5 78.60 ± 55.28e 0e 0 5.24 ± 7.29 H = 11.22, df = 3, P = 0.01
 24 dpi ConA0 9.97 ± 13.97f 2.05 ± 1.54f 0 0 H = 8.44, df = 3, P = 0.04
ConA5 13.27 ± 16.80 1.81 ± 2.06 32.85 ± 37.08 38.77 ± 52.46 H = 2.60, df = 3, P = 0.45
IFN-γ
 8 dpi ConA0 0 0 0 0 P = 1
ConA5 0 0 0 0 P = 1
 16 dpi ConA0 0 24.0 ± 53.66 0 0 H = 3.00, df = 3, P = 0.39
ConA5 0 0 0 0 P = 1
 24 dpi ConA0 0 0 0 0 P = 1
ConA5 594.21 ± 772.32g 0 939.29 ± 768.24g 546.31 ± 738.50 H = 8.80, df = 3, P = 0.03
  1. aP ≤ 0.02 for the significance of difference A vs AS (Mann-Whitney U-test, U = 22.5)
  2. bP ≤ 0.05 for the significance of difference A vs C (Mann-Whitney U-test, U = 22)
  3. cP ≤ 0.01 for the significance of difference A vs AS (Mann-Whitney U-test, U = 16)
  4. dP ≤ 0.01 for the significance of difference AS vs CS (Mann-Whitney U-test, U = 25)
  5. eP ≤ 0.02 for the significance of difference A vs C (Mann-Whitney U-test, U = 22.5)
  6. fP ≤ 0.05 for the significance of difference A vs C (Mann-Whitney U-test, U = 13.5)
  7. gP ≤ 0.05 for the significance of difference A vs AS (Mann-Whitney U-test, U = 11)
  8. Abbreviations: Group A, immunocompetent infected group; Group C, immunocompetent control group; Group AS immunosuppressed infected group; Group CS, immunosuppressed non-infected group; AM, arithmetic mean; SD, standard deviation