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Table 3 Cytokine profile after incubation of lymphocytes from the spleen with different doses of concanavalin A, ConA (0 μg/ml; 5 μg/ml)

From: Changes in the immune system in experimental acanthamoebiasis in immunocompetent and immunosuppressed hosts

Parameter/group

 

Group A

(n = 10)

Group C

(n = 6)

Group AS

(n = 10)

Group CS

(n = 6)

Kruskal-Wallis test

IL-4

 8 dpi

ConA0

0

0

0

0

P = 1.0

ConA5

0.52 ± 4.33

0.26 ± 0.59

0

0

H = 2.16, df = 3, P = 0.54

 16 dpi

ConA0

0

0

0

1.94 ± 4.33

H = 3.18, df = 3, P = 0.39

ConA5

50.35 ± 51.75a

0

0a

0

H = 11.22, df = 3, P = 0.002

 24 dpi

ConA0

0

0

1.28 ± 2.86

0

H = 3.00, df = 3, P = 0.31

ConA5

1.19 ± 2.67

0.66 ± 1.47

0

0

H = 21.00, df = 3, P = 0.54

IL-10

 8 dpi

ConA0

84.63 ± 46.72

149.98 ± 128.88

83.06 ± 37.32

58.90 ± 26.14

H = 3.94, df = 3, P = 0.27

ConA5

168.43 ± 180.88

212.92 ± 180.20

139.47 ± 125.42

86.16 ± 12.56

H = 4.07, df = 3, P = 0.25

 16 dpi

ConA0

128.27 ± 65.83

78.98 ± 58.82

78.60 ± 46.47

69.07 ± 15.19

H = 4.62, df = 3, P = 0.34

ConA5

511.09 ± 40.41b, c

67.42 ± 16.98b

276.18 ± 224.72c,d

81.01 ± 19.34d

H = 10.30, df = 3, P = 0.01

 24 dpi

ConA0

14.94 ± 33.40

19.99 ± 99.96

0

0

H = 2.16, df = 3, P = 0.55

ConA5

82.13 ± 82.73

36.54 ± 74.12

0

230.67 ± 515.76

H = 3.73, df = 3, P = 0.29

IL-17 A

 8 dpi

ConA0

0

0

0

0

P = 1.0

ConA5

13.86 ± 30.98

0

0

0

H = 3.00, df = 3, P = 0.39

 16 dpi

ConA0

0

0

0

0.75 ± 1.68

H = 4.00, df = 3, P = 0.56

ConA5

78.60 ± 55.28e

0e

0

5.24 ± 7.29

H = 11.22, df = 3, P = 0.01

 24 dpi

ConA0

9.97 ± 13.97f

2.05 ± 1.54f

0

0

H = 8.44, df = 3, P = 0.04

ConA5

13.27 ± 16.80

1.81 ± 2.06

32.85 ± 37.08

38.77 ± 52.46

H = 2.60, df = 3, P = 0.45

IFN-γ

 8 dpi

ConA0

0

0

0

0

P = 1

ConA5

0

0

0

0

P = 1

 16 dpi

ConA0

0

24.0 ± 53.66

0

0

H = 3.00, df = 3, P = 0.39

ConA5

0

0

0

0

P = 1

 24 dpi

ConA0

0

0

0

0

P = 1

ConA5

594.21 ± 772.32g

0

939.29 ± 768.24g

546.31 ± 738.50

H = 8.80, df = 3, P = 0.03

  1. aP ≤ 0.02 for the significance of difference A vs AS (Mann-Whitney U-test, U = 22.5)
  2. bP ≤ 0.05 for the significance of difference A vs C (Mann-Whitney U-test, U = 22)
  3. cP ≤ 0.01 for the significance of difference A vs AS (Mann-Whitney U-test, U = 16)
  4. dP ≤ 0.01 for the significance of difference AS vs CS (Mann-Whitney U-test, U = 25)
  5. eP ≤ 0.02 for the significance of difference A vs C (Mann-Whitney U-test, U = 22.5)
  6. fP ≤ 0.05 for the significance of difference A vs C (Mann-Whitney U-test, U = 13.5)
  7. gP ≤ 0.05 for the significance of difference A vs AS (Mann-Whitney U-test, U = 11)
  8. Abbreviations: Group A, immunocompetent infected group; Group C, immunocompetent control group; Group AS immunosuppressed infected group; Group CS, immunosuppressed non-infected group; AM, arithmetic mean; SD, standard deviation