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Fig. 1 | Parasites & Vectors

Fig. 1

From: Enhancement of insect susceptibility and larvicidal efficacy of Cry4Ba toxin by calcofluor

Fig. 1

Enhancing effect of calcofluor on the toxicity of wild-type Cry4Ba toward Ae. aegypti larvae. Larvae were fed with various concentrations of wild-type Cry4Ba toxin in the presence (+) or absence (-) of calcofluor for 24 h, LC50 and LC90 were estimated (a). The larvae were fed with toxin at LC90 alone (dash line), and toxin with calcofluor (solid line) and percent mortality was recorded after 3 h, 4 h, 6 h, 12 h and 24 h incubation period (b). Larvae were pre-incubated with 0.1% calcofluor for 0.25 h, 0.5 h, 1 h, 1.5 h and 2 h before 24 h feeding with toxin at LC90, and percent mortality was then recorded (c). Larvae were pre-exposed with 0.1% calcofluor for 2 h, and then the solution was replaced with toxin inclusion at LC90. Percent mortality was recorded after 3 h, 6 h and 12 h and presented as percent mortality + SEM (as T-bars) (d). Comparison of toxicity of Cry4Ba toxin toward larvae, which were 2 h pre-exposed with 0.1% calcofluor and continuously fed with the calcofluor together with LC90 of the toxin for 3 h, 6 h, and 12 h, and represented as folds of toxicity compared to calcofluor untreated larvae (e). Abbreviations: SEM, standard error of the mean

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