Fig. 2From: Enhancement of insect susceptibility and larvicidal efficacy of Cry4Ba toxin by calcofluorToxicity and biological activities of EY mutant toxin in the presence and absence of calcofluor. Larvae were fed with toxin inclusion bodies of wild-type Cry4Ba and EY mutant toxin at 5, 25 and 50 μg/ml, E. coli cells expressing Cry4Ba, and E. coli cells harbouring expressing plasmid pUC12. Percent mortality was represented as the mean + SEM (a). PM perturbation ability of Cry4Ba toxin was evaluated by feeding the larvae with PM impermeable 2000 kDa dextran (conjugated with FITC) alone or in combination with wild-type Cry4Ba and EY mutant toxin at their LC90 concentrations. Percent PM permeability was represented as % of larvae with fluorescent signal outside the gut lumen after one h treatment (b). The tissue sections of treated larvae were immuno-stained with mouse anti-Cry4Ba monoclonal antibody and HRP-linked anti-mouse immunoglobulins (c). LC50 and LC90 of EY mutant toxin were determined after 24 h treatment in the presence or absence of calcofluor and presented as μg/ml ± SEM (d). The lines point out the BBMV that are positively stained with wild-type Cry4Ba and EY mutant toxins. Control is untreated larval gut tissue. Abbreviations: L, lumen; BBMV, brush border microvilliBack to article page