Immune responses of Aedes togoi, Anopheles paraliae and Anopheles lesteri against nocturnally subperiodic Brugia malayi microfilariae during migration from the midgut to the site of development

Table 4 Comparisons of average numbers of normal and abnormal mf (degeneration and melanisation) of B. malayi from three parts [the midgut, haemocoel (haemolymph) and thoracic musculature] of Ae. togoi (AT), An. lesteri (AL) and An. paraliae (AP) at different time points post-infection (PI)

Time PI	Strain	Total no. of mf^a	No. of normal mf	H-value^b	P-value^c	No. of abnormal mf	H-value^b	P-value^c
5 min	AT	132	131	0.539		1	nd
	AL	194	194			0
	AP	161	161			0
1 h	AT	270	270	0.03	AP vs AT, P = 0.033	0	nd
	AL	256	256			0
	AP	100	100			0
2 h	AT	97	97	0.074		0	nd
	AL	244	243			1
	AP	105	105			0
3 h	AT	186	182	0.285		4	0.178
	AL	338	338			0
	AP	182	178			4
4 h	AT	188	187	0.18		1	0.162
	AL	199	198			1
	AP	130	124			6
5 h	AT	222	220	0.364		2	0.461
	AL	313	309			4
	AP	131	123			8
6 h	AT	88	87	0.571		1	0.182
	AL	311	306			5
	AP	111	102			9
12 h	AT	72	69	0.146		3	0.005	AT vs AP, P = 0.005
	AL	173	165			8
	AP	113	82			31
18 h	AT	19	19	0.02	AT vs AL, P = 0.021	0	0.006	AT vs AP, P = 0.005
	AL	298	282			16
	AP	254	215			39
24 h	AT	24	22	0.017	AT vs AL, P = 0.013	2	0.002	AT vs AP, P = 0.01
	AL	158	143			15
	AP	132	64			68
48 h	AT	72	57	0.092		15	0.165
	AL	38	21			17
	AP	44	11			33
72 h	AT	74	24	0.043	AP vs AL, P = 0.04	50	0.52
	AL	92	64			28
	AP	39	10			29

ISSN: 1756-3305