Table 7 Distribution of normal and abnormal mf (degeneration and melanisation) of B. malayi from the midgut, haemocoel (haemolymph) and thoracic musculature of An. paraliae at different time points post-infection (PI)
Time PI | Mean no. of total mf per infected midgut (range) | Mean no. of total mf per infected haemocoel (range) | Mean no. of total mf per infected thorax (range) | Normal and abnormal mf found in each part of mosquitoesa | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Midguta | Hemocoela | Thoraxa | ||||||||||
Mean no. of normal mf (%) | Mean no. of degenerated mf (%) | Mean no. of melanised mf (%) | Mean no. of normal mf (%) | Mean no. of degenerated mf (%) | Mean no. of melanised mf (%) | Mean no. of normal mf (%) | Mean no. of degenerated mf (%) | Mean no. of melanised mf (%) | ||||
5 min | 32.2 (15–49) | 0 (0) | 0 (0) | 32.2 (100) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
1 h | 17.4 (6–43) | 1.4 (0–7) | 1.2 (0–2) | 17.4 (100) | 0 (0) | 0 (0) | 1.4 (100) | 0 (0) | 0 (0) | 1.2 (100) | 0 (0) | 0 (0) |
2 h | 12.8 (5–26) | 4 (0–9) | 4.2 (2–8) | 12.8 (100) | 0 (0) | 0 (0) | 4 (100) | 0 (0) | 0 (0) | 4.2 (100) | 0 (0) | 0 (0) |
3 h | 15.6 (0–30) | 7.2 (0–24) | 13.6 (0–32) | 15.2 (97) | 0.4 (3) | 0 (0) | 6.8 (94) | 0.4 (6) | 0 (0) | 13.6 (100) | 0 (0) | 0 (0) |
4 h | 11.2 (0–16) | 6.8 (0–9) | 8 (4–14) | 10.8 (96) | 0.4 (4) | 0 (0) | 6 (88) | 0.2 (3) | 0.6 (9) | 8 (100) | 0 (0) | 0 (0) |
5 h | 8.6 (0–13) | 3.8 (0–5) | 13.8 (9–17) | 8 (93) | 0.6 (7) | 0 (0) | 2.8 (74) | 0.2 (5) | 0.8 (21) | 13.8 (100) | 0 (0) | 0 (0) |
6 h | 7.4 (3–10) | 5.8 (0–12) | 9 (1–18) | 7.4 (100) | 0 (0) | 0 (0) | 4 (69) | 0 (0) | 1.8 (31) | 9 (100) | 0 (0) | 0 (0) |
12 h | 3.2 (0–4) | 4.2 (0–9) | 15.2 (0–28) | 0.8 (24) | 1.2 (38) | 1.2 (38) | 1.8 (43) | 1.8 (43) | 0.6 (14) | 13.8 (91) | 1.4 (9) | 0 (0) |
18 h | 5.8 (0–8) | 11.6 (0–23) | 33.4 (0–63) | 3 (52) | 2.8 (48) | 0 (0) | 8.6 (74) | 2.2 (19) | 0.8 (7) | 31.4 (94) | 1.8 (5) | 0.2 (1) |
24 h | 7 (0–11) | 5.2 (0–7) | 14.2 (0–22) | 0.6 (9) | 4 (57) | 2.4 (34) | 1.6 (31) | 0.8 (15) | 2.8 (54) | 10.6 (75) | 3 (21) | 0.6 (4) |
48 h | 0.4 (0–2) | 3 (0–15) | 5.4 (0–5) | 0.4 (100) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 3 (100) | 1.8 (33) | 3.2 (60) | 0.4 (7) |
72 h | 0 (0) | 1.6 (0–3) | 6.2 (0–9) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0.2 (12) | 1.4 (88) | 2 (32) | 4.2 (68) | 0 (0) |