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Table 7 Distribution of normal and abnormal mf (degeneration and melanisation) of B. malayi from the midgut, haemocoel (haemolymph) and thoracic musculature of An. paraliae at different time points post-infection (PI)

From: Immune responses of Aedes togoi, Anopheles paraliae and Anopheles lesteri against nocturnally subperiodic Brugia malayi microfilariae during migration from the midgut to the site of development

Time PI Mean no. of total mf per infected midgut (range) Mean no. of total mf per infected haemocoel (range) Mean no. of total mf per infected thorax (range) Normal and abnormal mf found in each part of mosquitoesa
Midguta Hemocoela Thoraxa
Mean no. of normal mf (%) Mean no. of degenerated mf (%) Mean no. of melanised mf (%) Mean no. of normal mf (%) Mean no. of degenerated mf (%) Mean no. of melanised mf (%) Mean no. of normal mf (%) Mean no. of degenerated mf (%) Mean no. of melanised mf (%)
5 min 32.2 (15–49) 0 (0) 0 (0) 32.2 (100) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0)
1 h 17.4 (6–43) 1.4 (0–7) 1.2 (0–2) 17.4 (100) 0 (0) 0 (0) 1.4 (100) 0 (0) 0 (0) 1.2 (100) 0 (0) 0 (0)
2 h 12.8 (5–26) 4 (0–9) 4.2 (2–8) 12.8 (100) 0 (0) 0 (0) 4 (100) 0 (0) 0 (0) 4.2 (100) 0 (0) 0 (0)
3 h 15.6 (0–30) 7.2 (0–24) 13.6 (0–32) 15.2 (97) 0.4 (3) 0 (0) 6.8 (94) 0.4 (6) 0 (0) 13.6 (100) 0 (0) 0 (0)
4 h 11.2 (0–16) 6.8 (0–9) 8 (4–14) 10.8 (96) 0.4 (4) 0 (0) 6 (88) 0.2 (3) 0.6 (9) 8 (100) 0 (0) 0 (0)
5 h 8.6 (0–13) 3.8 (0–5) 13.8 (9–17) 8 (93) 0.6 (7) 0 (0) 2.8 (74) 0.2 (5) 0.8 (21) 13.8 (100) 0 (0) 0 (0)
6 h 7.4 (3–10) 5.8 (0–12) 9 (1–18) 7.4 (100) 0 (0) 0 (0) 4 (69) 0 (0) 1.8 (31) 9 (100) 0 (0) 0 (0)
12 h 3.2 (0–4) 4.2 (0–9) 15.2 (0–28) 0.8 (24) 1.2 (38) 1.2 (38) 1.8 (43) 1.8 (43) 0.6 (14) 13.8 (91) 1.4 (9) 0 (0)
18 h 5.8 (0–8) 11.6 (0–23) 33.4 (0–63) 3 (52) 2.8 (48) 0 (0) 8.6 (74) 2.2 (19) 0.8 (7) 31.4 (94) 1.8 (5) 0.2 (1)
24 h 7 (0–11) 5.2 (0–7) 14.2 (0–22) 0.6 (9) 4 (57) 2.4 (34) 1.6 (31) 0.8 (15) 2.8 (54) 10.6 (75) 3 (21) 0.6 (4)
48 h 0.4 (0–2) 3 (0–15) 5.4 (0–5) 0.4 (100) 0 (0) 0 (0) 0 (0) 0 (0) 3 (100) 1.8 (33) 3.2 (60) 0.4 (7)
72 h 0 (0) 1.6 (0–3) 6.2 (0–9) 0 (0) 0 (0) 0 (0) 0 (0) 0.2 (12) 1.4 (88) 2 (32) 4.2 (68) 0 (0)
  1. aDissected from five infected mosquitoes per time point