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Table 4 In vitro effects of esculetin (aglycone of esculin) on trehalase activities of female Lutzomyia longipalpis adults. Insects were fed on sucrose only, and esculetin was added in the assay mixtures of different tissues with trehalose as substrate. Tissues were homogenized and separated by centrifugation into gut tissue, gut contents, rest of body tissues and rest of body soluble fraction. Results are presented as μU/insect and are the mean and SEM of 6 biological replicates with 4 insects in each sample

From: Transmission blocking sugar baits for the control of Leishmania development inside sand flies using environmentally friendly beta-glycosides and their aglycones

Sample Control (trehalose only) Esculetin added to the assay mixture
Gut, tissue 290 ± 30 180 ± 60
Gut, contents 190 ± 50 110 ± 30
Rest of body, tissues 400 ± 30a 160 ± 40a
Rest of body, soluble 300 ± 30 330 ± 50
  1. aMann-Whitney test, U = 4, P = 0.026