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Table 6 In vitro effects of esculetin on the β-glucosidase activities of male Lutzomyia longipalpis. Tissue homogenates were incubated with esculetin and then assayed using cellobiose as a substrate. Tissues were homogenized and separated by centrifugation into gut tissue, gut contents, rest of body tissues and rest of body soluble fraction. Results are presented as μU/insect and are the mean and SEM of 4 biological replicates with 20 insects in each sample

From: Transmission blocking sugar baits for the control of Leishmania development inside sand flies using environmentally friendly beta-glycosides and their aglycones

Sample Control (cellobiose only) Esculetin added to the assay mixture
Gut, tissue 7 ± 3 0
Gut, contents 14 ± 3 11 ± 4
Rest of body, tissues 4 ± 1 4± 3
Rest of body, soluble 6 ± 2a 20 ± 10a
  1. aMann-Whitney test, U = 0, P = 0.0286