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Table 7 Quantification of the ingested esculin by Lutzomyia longipalpis adults exposed to esculin-supplemented diets. Flies were maintained on an esculin-supplemented sucrose diet for 6 to 7 days. Insects were immersed and homogenized in two different solvents, water or methanol. In the solvent test, flies were immersed for 8 days. Stability of fluorescence was evaluated by immersing esculin-fed insects in methanol and then homogenizing for fluorescence measurements after 1, 14 or 28 days. Basal fluorescence of the negative controls (sucrose-fed only) was subtracted from experimental sample values. Each sample used for extraction and measurement consisted of an individual sand fly. Results are shown in pmol/insect and are the mean and SEM of 3 biological replicates, with a total measurement of n = 22 (male) and n = 24 (female) in solvent tests and n = 29 (male) and n = 36 (female) in stability tests

From: Transmission blocking sugar baits for the control of Leishmania development inside sand flies using environmentally friendly beta-glycosides and their aglycones

Solvent Incubation time in the solvent (days) Males (pmol/insect) Females (pmol/insect)
Water 8 58 ± 8 67 ± 9
Methanol 8 52 ± 7 70 ± 7
Methanol 1 34 ± 4a 53 ± 5
Methanol 14 49 ± 5 47 ± 6
Methanol 28 49 ± 5 52 ± 7
  1. aMann-Whitney test, U = 106.5 P = 0.01