Table 8 Quantification of the esculin amount in different tissues of Lutzomyia longipalpis after exposure to esculin-supplemented diets. Insect adults were fed ad libitum on sucrose plus esculin for 7 days. After that, the flies were immersed individually in methanol and tissues were dissected and homogenized on the same day of collection from the cage. Basal fluorescence of the negative controls (sucrose-fed only) was subtracted from experimental sample values. Results are shown in pmol/insect and are the mean and SEM of 3 biological replicates with a total of n = 36 (male) and n = 29 (female)