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Fig. 1 | Parasites & Vectors

Fig. 1

From: Standardization and application of a modified RFLP-PCR methodology for analysis of polymorphisms linked to treatment resistance in Ancylostoma braziliense

Fig. 1

Representative RFLP-PCR results from the analysis of codons 167 (a), 198 (b) and 200 (c) of the Ancylostoma braziliense beta-tubulin gene using the restriction RsaI enzyme for codons 167 and 200, and the DdeI enzyme for codon 198. Lanes 1–3 contain undigested PCR products from plasmid controls (Lane 1: unmutated plasmid; Lane 2: mutated plasmid; Lane 3: mix of mutated and unmutated plasmid). Lanes 4–6 contain PCR digestion products from control plasmids (Lane 4: unmutated plasmid; Lane 5: mutated plasmid; Lane 6: mix of mutated and unmutated plasmid). Lanes 7–18 contain RFLP-PCR products from A. braziliense DNA. Each image is of a polyacrylamide gel (12%) stained with GelRed™ (Biotium, USA). Lane MW: 50 bp molecular weight ladder. Expected fragments sizes (bp): codon 167: unmutated: 162 + 95 + 49, mutated: 138 + 95 + 49 + 24; codon 198: unmutated: 186 + 28, mutated: 214; codon 200: unmutated: 137 + 41, mutated: 137 + 71 + 30

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Parasites & Vectors

ISSN: 1756-3305

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