Fig. 5
From: Editing the genome of Aphanomyces invadans using CRISPR/Cas9
Dwarf gourami showing necrotic ulceration after infection with the zoospores of A. invadans. Zoospore suspension (0.1 ml) coniatining 10,000 zoospores/ml of A. invadans was injected intramuscularly. a Positive control fish showing the reddening of the infected area (arrowhead) and the beginning of ulcer formation (arrow) at the site of infection at 3 dpi. b Positive control fish showing clear oomycete growth and necrotic ulceration (arrow) at the site of infection at 7 dpi. c Fish injected with RNP-2-treated A. invadans zoospores showing no clinical signs at 7 dpi. d Negative control fish at 7 dpi. Scale-bar: 1 cm