From: Editing the genome of Aphanomyces invadans using CRISPR/Cas9
gRNA id | gRNA sequence (PAM “NGG”) | Total score | Efficiency score | On-target hits in the genome (perfect-match | non-perfect-but-PAM-match) | Off-target hits (perfect-match | nonperfect-match) |
---|---|---|---|---|---|
1. Aphanomyces-invadans_106 | GCT TAC CAG ATG GAA TGA CG | TGG | 0.69 | 0.65 | 2 | 0 | 0 | 0 |
2. Aphanomyces-invadans_923 | GTG TAG CCC CAG AAG CCC AA | TGG | 0.56 | 0.57 | 1 | 1 | 0 | 0 |
3. Aphanomyces-invadans_1730 | GAT GGA ACC CAA CAA CTG TG | TGG | 0.53 | 0.65 | 1 | 0 | 0 | 0 |