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Table 2 Guide RNAs used in this study. The Eukaryotic Pathogen CRISPR Guide RNA Design Tool was used to design the gRNAs for serine type protease of A. invadans (gene ID: 20088162). Based on their high total and efficiency scores and zero off-target hits, three gRNAs were selected for genome modification experiment

From: Editing the genome of Aphanomyces invadans using CRISPR/Cas9

gRNA id gRNA sequence (PAM “NGG”) Total score Efficiency score On-target hits in the genome (perfect-match | non-perfect-but-PAM-match) Off-target hits (perfect-match | nonperfect-match)
1. Aphanomyces-invadans_106 GCT TAC CAG ATG GAA TGA CG | TGG 0.69 0.65 2 | 0 0 | 0
2. Aphanomyces-invadans_923 GTG TAG CCC CAG AAG CCC AA | TGG 0.56 0.57 1 | 1 0 | 0
3. Aphanomyces-invadans_1730 GAT GGA ACC CAA CAA CTG TG | TGG 0.53 0.65 1 | 0 0 | 0
  1. Abbreviation: PAM protospacer adjacent motif