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Table 2 Guide RNAs used in this study. The Eukaryotic Pathogen CRISPR Guide RNA Design Tool was used to design the gRNAs for serine type protease of A. invadans (gene ID: 20088162). Based on their high total and efficiency scores and zero off-target hits, three gRNAs were selected for genome modification experiment

From: Editing the genome of Aphanomyces invadans using CRISPR/Cas9

gRNA id

gRNA sequence (PAM “NGG”)

Total score

Efficiency score

On-target hits in the genome (perfect-match | non-perfect-but-PAM-match)

Off-target hits (perfect-match | nonperfect-match)

1. Aphanomyces-invadans_106

GCT TAC CAG ATG GAA TGA CG | TGG

0.69

0.65

2 | 0

0 | 0

2. Aphanomyces-invadans_923

GTG TAG CCC CAG AAG CCC AA | TGG

0.56

0.57

1 | 1

0 | 0

3. Aphanomyces-invadans_1730

GAT GGA ACC CAA CAA CTG TG | TGG

0.53

0.65

1 | 0

0 | 0

  1. Abbreviation: PAM protospacer adjacent motif