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Fig. 3 | Parasites & Vectors

Fig. 3

From: Exploiting genetic polymorphisms in metabolic enzymes for rapid screening of Leishmania infantum genotypes

Fig. 3

HRM analysis of amplicons with genotypes 390G and 390T. a HRM profiles obtained from qPCR-MEint amplicons of strains MHOM/TN/80/IPT1 and MHOM/DZ/82/LIPA59 (genotype 390G) and amplicons of strains MHOM/FR/78/LEM75 and MHOM/IT/86/ISS218 (genotype 390T). b The analysis of HRM profiles allowed the assignation of amplicons of clinical isolates 1 and 2 to genotypes 390G and 390T, respectively, with a confidence > 95% in at least one replicate. Strains MHOM/DZ/82/LIPA59 (genotype 390G) and MHOM/FR/78/LEM75 (genotype 390T) were used as reference. Analyses were performed in duplicates

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