Fig. 5From: The pervasive effects of recombinant Fasciola gigantica Ras-related protein Rab10 on the functions of goat peripheral blood mononuclear cellsrFgRab10 protein induced apoptosis in PBMCs. Annexin V/PI staining combined with flow cytometry analysis was used to count apoptotic cells. a Dot plots showing apoptosis of PBMCs in response to exposure to rFgRab10 protein. b Percentage of cell population was compared with plotted apoptotic cells (Annexin V+/PI-). Graphs represent means ± SD of data from three independent biological replicates. Asterisks indicate statistical significance between rFgRab10-treated, SUMO protein and sham-treated PBMCs (****P < 0.0001; ns, non-significant). The statistical significance was only achieved at the two highest concentrations (40 μg/ml and 80 μg/ml)Back to article page