Fig. 3

LGTV infection of tick cells in vitro affects expression of isoatp2114, isoatp4548, isoatp4550 and isoatp5126. Data from QRT-PCR assays are shown in all panels. a-i Expression of nine I. scapularis OATPs: isoatp-0726 (a), -2114 (b), -2116 (c), -4056 (d), -4134 (e), -4548 (f), -4550 (g), -5126 (h), -5621 (i) and kat mRNA (j) in unfed uninfected ticks and LGTV-infected tick cells at 24 and 72 h pi. k Levels of viral RNA in LGTV-infected ISE6 tick cell line (LGTV) at 24 and 72 h pi. is shown. Uninfected (UI) cells were used as controls in all panels. In all panels, open circles represent data from uninfected (UI) and closed circles represent data from LGTV-infected (LGTV) tick cells. Each circle represents data from one cell culture well. LGTV loads or oatps or kat mRNA levels was normalized to the amount of tick beta-actin mRNA levels. The P-values indicate the results from statistical analyses